Oxidation of nicotinic acid by a Bacillus species: regulation of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
نویسندگان
چکیده
The first two enzymes employed by a Bacillus species for the dissimilation of nicotinic acid are coordinately induced. The inducer of the enzymes appears to be 6-hydroxynicotinic acid, the product of the first enzyme in the pathways. Synthesis of the enzymes is repressed by glucose when ammonium is present in the medium, but not when nicotinic acid is the sole nitrogen source. The possible significance of the coordinate induction and unusual repression is discussed.
منابع مشابه
Oxidation of nicotinic acid by a Bacillus species: purification and properties of nicotinic acid and 6-hydroxynicotinic acid hydroxylases.
The enzymes of a Bacillus species that hydroxylate nicotinic acid to 6-hydroxynicotinic acid and 6-hydroxynicotinic acid to 2,6-dihydroxynicotinic acid were purified and characterized. The purified enzymes contained approximately two molecules of flavine and eight molecules of iron per molecule of enzyme. The enzymes were large (molecular weight, 400,000 to 450,000) and appeared to consist of s...
متن کاملThe Pathway of Nicotinic Acid Oxidation by a Bacillus Species.
Bacteria able to grow with nicotinic acid as a sole source of carbon and nitrogen have been isolated by a number of investigators (l-5). Hughes et al. (6-10) found that Pseudomonas jlzwrescens initiates degradation of nicotinic acid by a hydroxylation to form 6-hydroxynicotinic acid and established that the responsible enzyme is located in a cell wall membrane fraction. An initial hydroxylation...
متن کاملOxidation of nicotinic acid by a Bacillus species: source of oxygen atoms for the hydroxylation of nicotinic acid and 6-hydroxynicotinic acid.
Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms. (18)O is incorporated into the products from H(2) (18)O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required...
متن کاملNicotinic acid metabolism. IV. Ferredoxin-dependent reduction of 6-hydroxynicotinic acid to 6-oxo-1,4,5,6-tetrahydronicotinic acid.
Previous reports from this laboratory have described the isolation of 6-hydroxynicotinic acid and 6-0x0-1 ,4,5,6-tetrahydronicotinic acid from the reaction media of a clostridial species grown on nicotinic acid (1). Bn enzyme that catalyzes the reversible oxidation of nicotinic acid to HNA’ has been purified from extracts of this microorganism (2). Since fortified crude extract of this Clostrid...
متن کاملBacterial Fermentation of Nicotinic Acid
The ability of certain bacterial species to oxidize nicotinic acid was first reported by Allison (1) who used suspensions of bacteria isolated by Dubos and Miller (2). Koser and Baird (3) later isolated strains of Pseudomonas jluorescens which grew on a synthetic medium in which nicotinic acid was the sole carbon source. These strains did not attack isonicotinic acid, picolinic acid, or pyridin...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 112 1 شماره
صفحات -
تاریخ انتشار 1972